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KMID : 0364819860240010012
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Korean Journal of Microbiology
1986 Volume.24 No. 1 p.12 ~ p.17
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Rapid Plasmid Mapping Computer Program
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Lee,Dong-Hun/이동훈
Kim,Young-Jun/Lee,Seung-Taek/Kang,Hyen-Sam/김영준/이승택/강현삼
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Abstract
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1
A new computer algorithm is described to order the restriction fragments of plasmid DNA which has been cleaved with several restriction endonucleases in single or double digestions rapidly with realistic error rates. The permutation and high weight on small fragments methods construct all logical circular map solutions. The program is written in Apple BASIC and run on an Apple II plus microcomputer with 64K memory. Several examples are presented which indicate the high efficiency of the program in constructing possible restriction map for YEp24.
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KEYWORD
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